rnaQUAST is a software designed for quality evaluation and assessment of de novo transcriptome assemblies. rnaQUAST is mainly intended for testing different assembly methods and pipelines on well-known organisms and provides the most informative reports when reference genome and gene database are provided. However, it allows to perform de novo evaluation using several third-party tools, such as BUSCO and GeneMarkS-T.

rnaQUAST manual (v2.2.2)
Download rnaQUAST 2.2.2 (finally supports Python3)

GitHub page