VarQuest is a novel algorithm for identification of PNP variants via database search of mass spectra, the first high-throughput mutation-tolerant PNP identification method capable of analysing the entire GNPS infrastructure. VarQuest is based on Dereplicator source code, a method aimed at standard PNP identification.

You can try VarQuest online at GNPS website (registration is needed but it is quick and simple). Our tool is embedded into Dereplicator workflow (use “Search analogs” checkbox to enable VarQuest). See documentation for details.

Examples of VarQuest output (interactive PSM viewers):

  1. Surugamide-783 identified in MSV000078604 data set.
  2. Surugamide-769 identified in MSV000078604 data set.
  3. Pristinamycin-733 identified in MSV000078604 data set.
  4. Venepeptide-2138 identified in MSV000078839 data set.
  5. Venepeptide-2154 identified in MSV000078839 data set.
  6. Wollamide-1017 identified in MSV000078604 data set.
  7. WS9326C-1036 identified in MSV000078604 data set.
  8. Massetolide-1252 identified in MSV000079450 data set.

The tool is developed in collaboration with University of California, San Diego.