Developing a novel assembler software clearly requires a quality evaluation tool that is capable to assess and compare various assembly approaches. An example of QUAST evaluation tool and SPAdes genome assembler show that parallel development appears to be beneficial for both tool. However, currently no tool has set a standard in assessing quality of assembled transcripts, which created a need of developing rnaQUAST — a quality evaluation tool for assembled transcripts. rnaQUAST is capable of utilizing reference genome and gene database, and at the same time allows to assemble transcripts de novo using several thrid-party tools, such as BUSCO and GeneMarkS-T.
rnaQUAST: a quality assessment tool for de novo transcriptome assemblies. Bioinformatics, 2016